Otago Harbour (Port Code: NZORR) is the natural harbour of Dunedin, New Zealand, consisting of a long, much-indented stretch of generally navigable water separating the Otago Peninsula from the mainland. They join at its southwest end, 21 km (13 mi) from the harbour mouth. It is home to Dunedin's two port facilities, Port Chalmers (half way along the harbour) and at Dunedin's wharf (at the harbour's end). The harbour has been of significant economic importance for approximately 700 years, as a sheltered harbour and fishery, then deep water port.
Substantial container port facilities exist at Port Chalmers, 9 km (6 mi) along the western shore from the harbour mouth. A channel along the western side of the harbour is regularly dredged, allowing vessels with a draught of 12.5 m to Port Chalmers, and 8 m all the way to the heart of Dunedin. New Zealand's frozen meat export trade was inaugurated at Port Chalmers in 1882. Portobello is home to one of New Zealand's leading marine research establishments, the Portobello Marine Laboratory, a department of the University of Otago. This lies at the harbour's edge on the tip of a small arm of the Otago Peninsula, the Portobello Peninsula. There are currently numerous sightseeing and fishing boats that can be chartered and one scheduled ferry from Port Chalmers to Portobello.